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• ### Exploring the March to Mars Using 3D Print Models

Learning Objectives
• Students will be able to describe the major aspects of the Mars Curiosity Rover missions.
• Students will be able to synthesize information learned from a classroom jigsaw activity on the Mars Curiosity Rover missions.
• Students will be able to work in teams to plan a future manned mission to Mars.
• Students will be able to summarize their reports to the class.
• ### Your Tax Dollars at Work: A mock grant writing experience centered on scientific process skills

Learning Objectives
Students will be able to:
• Propose a testable, novel question contributing to a biological field of study.
• Formulate a study rationale.
• Describe relevant background information on a topic using the primary literature.
• Choose appropriate scientific, mathematical, and statistical methods to analyze a research question.
• Determine the financial costs of a research project.
• Present a proposal for peer review and compose a constructive peer review.
• Collaborate as a member of a scientific team.
• Articulate the review criteria and process used in NSF-style proposal review.
• ### A first lesson in mathematical modeling for biologists: Rocs

Learning Objectives
• Systematically develop a functioning, discrete, single-species model of an exponentially-growing or -declining population.
• Use the model to recommend appropriate action for population management.
• Communicate model output and recommendations to non-expert audiences.
• Generate a collaborative work product that most individuals could not generate on their own, given time and resource constraints.
• ### Investigating the Function of a Transport Protein: Where is ABCB6 Located in Human Cells?

Learning Objectives
At the end of this activity students will be able to:
• describe the use of two common research techniques for studying proteins: SDS-PAGE and immunoblot analysis.
• determine a protein’s subcellular location based on results from: 1) immunoblotting after differential centrifugation, and 2) immunofluorescence microscopy.
• analyze protein localization data based on the limitations of differential centrifugation and immunofluorescence microscopy.
• ### Teaching the Biological Relevance of Chemical Kinetics Using Cold-Blooded Animal Biology

Learning Objectives
Students will be able to:
• Predict the effect of reaction temperature on the rate of a chemical reaction
• Interpret a graph plotted between rate of a chemical reaction and temperature
• Discuss chemical kinetics utilizing case studies of cold-blooded animals
• ### Does it pose a threat? Investigating the impact of Bt corn on monarch butterflies

Learning Objectives
Students will be able to:
• Apply genetics concepts to a relevant case study of Bt corn and monarch butterflies
• Read figures and text from primary literature
• Identify claims presented in scientific studies
• Evaluate data presented in scientific studies
• Critically reason using data
• Evaluate the consequences of GM technology on non-target organisms
• Communicate scientific data orally
• ### In-class peer grading of daily quizzes increases feedback opportunities

Learning Objectives
Each of these objectives are illustrated with a succinct slide presentation or other supplemental material available ahead of class time through the course administration system. Learners found it particularly helpful to have video clips that remind them of mathematical manipulations available (in the above example objective c). Students understand that foundational objectives tend to be the focus of the quiz (objectives a-d) and that others will be given more time to work on together in class (objectives e-g), but I don't specify this exactly to reduce temptation that 'gamers' take a shortcut that would impact their group work negatively later on. However, the assignment for a focused graded group activity is posted as well, so it is clear what we are working towards; if desired individuals could prepare ahead of the class.
• ### Discovery Poster Project

Learning Objectives
Students will be able to:
• identify and learn about a scientific research discovery of interest to them using popular press articles and the primary literature
• find a group on campus doing research that aligns with their interests and communicate with the faculty leader of that group
• create and present a poster that synthesizes their knowledge of the research beyond the discovery
• ### Infectious Chocolate Joy with a Side of Poissonian Statistics: An activity connecting life science students with subtle...

Learning Objectives
• Students will define a Poisson distribution.
• Students will generate a data set on the probability of a T cell being infected with a virus(es).
• Students will predict the likelihood of one observing the mean value of viruses occurring.
• Students will evaluate the outcomes of a random process.
• Students will hypothesize whether a process is Poissonian and design a test for that hypothesis.
• Students will collect data and create a histogram from their data.
• ### Sex-specific differences in Meiosis: Real-world applications

Learning Objectives
After completion of the lesson students will be able to:
1. Describe the differences between female and male meiosis.
2. Interpret graphical data to make decisions relevant to medical practices.
3. Develop a hypothesis that explains the difference in incidence of aneuploidy in gametes between males and females.
• ### Using Undergraduate Molecular Biology Labs to Discover Targets of miRNAs in Humans

Learning Objectives
• Use biological databases to generate and compare lists of predicted miR targets, and obtain the mRNA sequence of their selected candidate gene
• Use bioinformatics tools to design and optimize primer sets for qPCR
• ### Dynamic Daphnia: An inquiry-based research experience in ecology that teaches the scientific process to first-year...

Learning Objectives
Students will be able to:
• Construct written predictions about 1 factor experiments.
• Interpret simple (2 variables) figures.
• Construct simple (2 variables) figures from data.
• Design simple 1 factor experiments with appropriate controls.
• Demonstrate proper use of standard laboratory items, including a two-stop pipette, stereomicroscope, and laboratory notebook.
• Calculate means and standard deviations.
• Given some scaffolding (instructions), select the correct statistical test for a data set, be able to run a t-test, ANOVA, chi-squared test, and linear regression in Microsoft Excel, and be able to correctly interpret their results.
• Construct and present a scientific poster.
• ### Air Quality Data Mining: Mining the US EPA AirData website for student-led evaluation of air quality issues

Learning Objectives
Students will be able to:
• Describe various parameters of air quality that can negatively impact human health, list priority air pollutants, and interpret the EPA Air Quality Index as it relates to human health.
• Identify an air quality problem that varies on spatial and/or temporal scales that can be addressed using publicly available U.S. EPA air data.
• Collect appropriate U.S. EPA Airdata information needed to answer that/those questions, using the U.S. EPA Airdata website data mining tools.
• Analyze the data as needed to address or answer their question(s).
• Interpret data and draw conclusions regarding air quality levels and/or impacts on human and public health.
• Communicate results in the form of a scientific paper.
• ### Promoting Climate Change Literacy for Non-majors: Implementation of an atmospheric carbon dioxide modeling activity as...

Learning Objectives
• Students will be able to manipulate and produce data and graphs.
• Students will be able to design a simple mathematical model of atmospheric CO2 that can be used to make predictions.
• Students will be able to conduct simulations, analyze, interpret, and draw conclusions about atmospheric CO2 levels from their own computer generated simulated data.

• ### Modeling the Research Process: Authentic human physiology research in a large non-majors course

Learning Objectives
Students will be able to:
• Formulate testable hypotheses
• Design an experimental procedure to test their hypothesis
• Make scientific observations
• Analyze and interpret data
• Communicate results visually and orally
• ### Learning to Pipet Correctly by Pipetting Incorrectly?

Learning Objectives
• Students will be able to use analytical balances and micropipettes.
• Students will be able to calculate averages and standard deviations.
• Students will be able to use t-tests to compare two independent samples.
• Students will be able to justify accepting or rejecting a null hypothesis based on an interpretation of p-values.
• Students will learn to use spreadsheet software such as Microsoft Excel and/or Google Sheets
• Students will be able to explain how pipetting incorrectly leads to errors.
• ### Discovering Prokaryotic Gene Regulation with Simulations of the trp Operon

Learning Objectives
Students will be able to:
• Perturb and interpret simulations of the trp operon.
• Define how simulation results relate to cellular events.
• Describe the biological role of the trp operon.
• Describe cellular mechanisms regulating the trp operon.
• Explain mechanistically how changes in the extracellular environment affect the trp operon.
• Define the impact of mutations on trp operon expression and regulation.

Learning Objectives
Students will:
• Articulate testable hypotheses. (Lab 8, final presentation/paper, in-class exercises)
• Analyze data to determine the level of support for articulated hypotheses. (Labs 4-7, final presentation/paper)
• Identify multiple species of plants in the field quickly and accurately. (Labs 2-3, field trip)
• Measure environmental variables and sample vegetation in the field. (Labs 2-3, field trip)
• Analyze soil samples using a variety of low-tech lab techniques. (Open labs after field trip)
• Use multiple statistical techniques to analyze data for patterns. (Labs 4-8, final presentation/paper)
• Interpret statistical analyses to distinguish between strong and weak interactions in a biological system. (Labs 4-7, final presentation/paper)
• Develop and present a conference-style presentation in a public forum. (Lab 8, final presentation/paper)
• Write a publication-ready research paper communicating findings and displaying data. (Lab 8, final presentation/paper)
• ### Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Genetics (analyzing mutant...

Learning Objectives
• Describe how cells can produce proteins at the right time and correct amount.
• Diagram a bacterial promoter with −35 and −10 elements and the transcription start site.
• Describe how mutational analysis can be used to study promoter sequence requirements.
• Develop a promoter mutation hypothesis and design an experiment to test it.
• Successfully and safely manipulate DNA and Escherichia coli for ligation and transformation experiments.
• Design an experiment to verify a mutated promoter has been cloned into a destination vector.
• Design an experiment to measure the strength of a promoter.
• Analyze data showing reporter protein produced and use the data to assess promoter strength.
• Define type IIs restriction enzymes.
• Distinguish between type II and type IIs restriction enzymes.
• Explain how Golden Gate Assembly (GGA) works.
• Measure the relative strength of a promoter compared to a standard promoter.
• ### Quantifying and Visualizing Campus Tree Phenology

Learning Objectives
The Learning Objectives of this lesson span across the entire semester.
• Observe and collect information on phenological changes in local trees.
• Become familiar with a database and how to work with large datasets.
• Analyze and visualize data from the database to test their hypotheses and questions.
• Develop a research proposal including empirically-driven questions and hypotheses.
• Synthesize the results of their analysis in the context of plant biodiversity and local environmental conditions.
• ### The ACTN3 Polymorphism: Applications in Genetics and Physiology Teaching Laboratories

Learning Objectives
1. Test hypotheses related to the role of ACTN3 in skeletal muscle function.
2. Explain how polymorphic variants of the ACTN3 gene affect protein structure and function.
3. List and explain the differences between fast twitch and slow twitch muscle fibers.
4. List and explain possible roles of the ACTN3 protein in skeletal muscle function.
5. Find and analyze relevant scientific publications about the relationship between ACTN3 genotype and muscle function.
6. Formulate hypotheses related to the relationship between ACTN3 genotype and skeletal muscle function.
7. Design experiments to test hypotheses about the role of ACTN3 in skeletal muscle function.
8. Statistically analyze experimental results using relevant software.
9. Present experimental results in writing.
• ### A flexible, multi-week approach to plant biology - How will plants respond to higher levels of CO2?

Learning Objectives
Students will be able to:
• Apply findings from each week's lesson to make predictions and informed hypotheses about the next week's lesson.
• Keep a detailed laboratory notebook.
• Write and peer-edit the sections of a scientific paper, and collaboratively write an entire lab report in the form of a scientific research paper.
• Search for, find, and read scientific research papers.
• Work together as a team to conduct experiments.
• Connect findings and ideas from each week's lesson to get a broader understanding of how plants will respond to higher levels of CO2 (e.g., stomatal density, photosynthetic/respiratory rates, foliar protein concentrations, growth, and resource allocation).
Note: Additional, more specific objectives are included with each of the four lessons (Supporting Files S1-S4)
• ### You and Your Oral Microflora: Introducing non-biology majors to their “forgotten organ”

Learning Objectives
Students will be able to:
• Explain both beneficial and detrimental roles of microbes in human health.
• Compare and contrast DNA replication as it occurs inside a cell versus in a test tube
• Identify an unknown sequence of DNA by performing a BLAST search
• Navigate sources of scientific information to assess the accuracy of their experimental techniques
• ### Why do Some People Inherit a Predisposition to Cancer? A small group activity on cancer genetics

Learning Objectives
At the end of this activity, we expect students will be able to:
1. Use family pedigrees and additional genetic information to determine inheritance patterns for hereditary forms of cancer
2. Explain why a person with or without cancer can pass on a mutant allele to the next generation and how that impacts probability calculations
3. Distinguish between proto-oncogenes and tumor suppressor genes
• ### Bad Science: Exploring the unethical research behind a putative memory supplement

Learning Objectives
Students will be able to:
• create criteria for evaluating information that is touted as scientific.
• apply those criteria to evaluate the claim that Prevagen® enhances memory.
• identify the misleading tactics used on the Prevagen® website and in their self-published reporting.
• decide whether to recommend taking Prevagen® and explain their decisions.
• ### Bad Cell Reception? Using a cell part activity to help students appreciate cell biology, with an improved data plan and...

Learning Objectives
• Identify cell parts and explain their function
• Explain how defects in a cell part can result in human disease
• Generate thought-provoking questions that expand upon existing knowledge
• Create a hypothesis and plan an experiment to answer a cell part question
• Find and reference relevant cell biology journal articles
• ### Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Introductory Biology (identifying...

Learning Objectives
• Describe how cells can produce proteins at the right time and correct amount.
• Diagram how a repressor works to reduce transcription.
• Diagram how an activator works to increase transcription.
• Identify a new promoter from literature and design a method to clone it and test its function.
• Successfully and safely manipulate DNA and Escherichia coli for ligation and transformation experiments.
• Design an experiment to verify a new promoter has been cloned into a destination vector.
• Design an experiment to measure the strength of a promoter.
• Analyze data showing reporter protein produced and use the data to assess promoter strength.
• Define type IIs restriction enzymes.
• Distinguish between type II and type IIs restriction enzymes.
• Explain how Golden Gate Assembly (GGA) works.
• Measure the relative strength of a promoter compared to a standard promoter.
• ### Teaching epidemiology and principles of infectious disease using popular media and the case of Typhoid Mary

Learning Objectives
Students will be able to:
• Describe the reservoirs of infection in humans.
• Distinguish portals of entry and exit.
• Describe how each of the following contributes to bacterial virulence: adhesins, extracellular enzymes, toxins, and antiphagocytic factors.
• Define and distinguish etiology and epidemiology.
• Describe the five typical stages of infectious disease and depict the stages in graphical form.
• Contrast contact, vehicle and vector transmission, biological and mechanical vectors and identify the mode of transmission in a given scenario.
• Differentiate endemic, sporadic, epidemic, and pandemic disease.
• Distinguish descriptive, analytical, and experimental epidemiology.
• Compare and contrast social, economic, and cultural factors impacting health care in the early 1900s and today.
• ### Using computational molecular modeling software to demonstrate how DNA mutations cause phenotypes

Learning Objectives
Students successfully completing this lesson will:
1. Practice basic molecular biology laboratory skills such as DNA isolation, PCR, and gel electrophoresis.
2. Gather and analyze quantitative and qualitative scientific data and present it in figures.
3. Use bioinformatics to analyze DNA sequences and obtain protein sequences for molecular modeling.
4. Make and analyze three-dimensional (3-D) protein models using molecular modeling software.
5. Write a laboratory report using the collected data to explain how mutations in the DNA cause changes in protein structure/function which lead to mutant phenotypes.
• ### The Leaky Neuron: Understanding synaptic integration using an analogy involving leaky cups

Learning Objectives
Students will able to:
• compare and contrast spatial and temporal summation in terms of the number of presynaptic events and the timing of these events
• predict the relative contribution to reaching threshold and firing an action potential as a function of distance from the axon hillock
• predict how the frequency of incoming presynaptic action potentials effects the success of temporal summation of resultant postsynaptic potentials
• ### CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students

Learning Objectives
Week 1: CRISPR design
• Locate the coding sequence, flanking sequence, protein product, and characteristics of a given gene from the Saccharomyces Genome Database (https://www.yeastgenome.org/).
• Design and defend the design of guide RNA and single stranded template for DNA repair in CRISPR/Cas9 gene editing studies to generate Saccharomyces cerevisiae auxotrophic mutants.
Week 3-4: Cloning
• Describe the qualities of the vector, pML104, that allow replication and selection in bacteria and yeast as well as allow expression of necessary factors in CRISPR/Cas9 genome editing, including Cas9 and sgRNA.
• Describe the rationale of and perform procedures necessary for cloning a small cassette (i.e., sgRNA gene) into a vector (i.e., pML104) including; restriction digest, annealing of DNA strands, removal of 5’ phosphates, ligation, and transformation.
• Recognize and design appropriate controls for cloning procedures such as ligation and transformation.
Week 5: Screening clones
• Describe the method of polymerase chain reaction (PCR), including the rationale for essential components of a reaction mixture and thermal-cycling conditions.
• Locate the binding sites of and design primers for PCR, then report the expected size of the amplification product.
• Describe and perform isolation of plasmid DNA from E. coli.
Week 6: Selection of clones and transformation of yeast
• Describe the rationale for and perform procedures to transform yeast, including the essential components of a transformation mixture and conditions necessary for transformation.
• Describe the basic conditions required for cultivating yeast.
• Describe the rationale for and perform agarose gel electrophoresis of a given size of DNA.
• Analyze DNA separated by agarose gel electrophoresis, including size estimation.
• Recognize and describe the qualities of a template for DNA repair that allows efficient DNA repair.
Week 7: Phenotyping
• Design an experiment to determine auxotrophic phenotypes.
• Predict the outcome of multi-step experiments.
Multiweek
• Recognize and describe conditions necessary for growth of E. coli and S. cerevisiae.
• Qualitatively and quantitatively analyze scientific data from scientific experiments, including bacterial and yeast transformation, agarose gel electrophoresis, extraction of plasmid DNA from bacteria, PCR, and auxotroph phenotypic analysis.
• Communicate science to peers through maintenance of a laboratory notebook, verbal communication with group members, and writing of a formal laboratory report written in a format acceptable for journal publication.
• Troubleshoot scientific protocols by identifying procedures that are prone to error, comparing recommended protocols to actual procedure, and using positive and negative controls to narrow the location of a potential error.
• Communicate specific potential or actual uses of CRISPR/Cas9 in science and/or medicine.
Alignment with Society-Generated Learning Objectives - From Biochemistry and Molecular Biology, and Genetics Learning Frameworks
• Use various bioinformatics approaches to analyze macromolecular primary sequence and structure.
• Illustrate how DNA is replicated and genes are transmitted from one generation to the next in multiple types of organisms including bacteria, eukaryotes, viruses, and retroviruses.
• Define what a genome consists of and how the information in various genes and other sequence classes within each genome are used to store and express genetic information.
• Explain the meaning of ploidy (haploid, diploid, aneuploid etc.) and how it relates to the number of homologues of each chromosome.
• Predict the effects of mutations on the activity, structure, or stability of a protein and design appropriate experiments to assess the effects of mutations.
• Predict the growth behavior of microbes based on their growth conditions, e.g., temperature, available nutrient, aeration level, etc.
• Discuss the benefits of specific tools of modern biotechnology that are derived from naturally occurring microbes (e.g. cloning vectors, restriction enzymes, Taq polymerase, etc.)
• Accurately prepare and use reagents and perform experiments.
• When presented with an observation, develop a testable and falsifiable hypothesis.
• When provided with a hypothesis, identify the appropriate experimental observations and controllable variables.
• ### A CURE-based approach to teaching genomics using mitochondrial genomes

Learning Objectives
• Install the appropriate programs such as Putty and WinSCP.
• Navigate NCBI's website including their different BLAST programs (e.g., blastn, tblastx, blastp and blastx)
• Use command-line BLAST to identify mitochondrial contigs within a whole genome assembly
• Filter the desired sequence (using grep) and move the assembled mitochondrial genome onto your own computer (using FTP or SCP)
• Error-correct contigs (bwa mem, samtools tview), connect and circularize organellar contigs (extending from filtered reads)
• Transform assembled sequences into annotated genomes
• Orient to canonical start locations in the mitochondrial genome (cox1)
• Identify the boundaries of all coding components of the mitochondrial genome using BLAST, including: Protein coding genes (BLASTx and tBLASTX), tRNAs (proprietary programs such as tRNAscan), rRNAs (BLASTn, Chlorobox), ORFs (NCBI's ORFFinder)
• Deposit annotation onto genome repository (NCBI)
• Update CV/resume to reflect bioinformatics skills learned in this lesson
• ### Building a Model of Tumorigenesis: A small group activity for a cancer biology/cell biology course

Learning Objectives
At the end of the activity, students will be able to:
• Analyze data from a retrospective clinical study uncovering genetic alterations in colorectal cancer.
• Draw conclusions about human tumorigenesis using data from a retrospective clinical study.
• Present scientific data in an appropriate and accurate way.
• Discuss why modeling is an important practice of science.
• Create a simple model of the genetic changes associated with a particular human cancer.
• ### A new approach to course-based research using a hermit crab-hydrozoan symbiosis

Learning Objectives
Students will be able to:
• define different types of symbiotic interactions, with specific examples.
• summarize and critically evaluate contemporary primary literature relevant to ecological symbioses, in particular that between hermit crabs and Hydractinia spp.
• articulate a question, based on observations of a natural phenomenon (in this example, the hermit crab-Hydractinia interaction).
• articulate a testable hypothesis, based on their own observations and read of the literature.
• design appropriate experimental or observational studies to address their hypotheses.
• collect and interpret data in light of their hypotheses.
• problem-solve and troubleshoot issues that arise during their experiment.
• communicate scientific results, both orally and in written form.
• ### Follow the Sulfur: Using Yeast Mutants to Study a Metabolic Pathway

Learning Objectives
At the end of this lesson, students will be able to:
• use spot plating techniques to compare the growth of yeast strains on solid culture media.
• predict the ability of specific met deletion strains to grow on media containing various sulfur sources.
• predict how mutations in specific genes will affect the concentrations of metabolites in the pathways involved in methionine biosynthesis.
• ### Discovering Cellular Respiration with Computational Modeling and Simulations

Learning Objectives
Students will be able to:
• Describe how changes in cellular homeostasis affect metabolic intermediates.
• Perturb and interpret a simulation of cellular respiration.
• Describe cellular mechanisms regulating cellular respiration.
• Describe how glucose, oxygen, and coenzymes affect cellular respiration.
• Describe the interconnectedness of cellular respiration.
• Identify and describe the inputs and outputs of cellular respiration, glycolysis, pyruvate processing, citric acid cycle, and the electron transport chain.
• Describe how different energy sources are used in cellular respiration.
• Trace carbon through cellular respiration from glucose to carbon dioxide.
• ### Inexpensive Cell Migration Inquiry Lab using Zebrafish

Learning Objectives
Students will:
• formulate a hypothesis and design an experiment with the proper controls.
• describe the steps involved in the zebrafish wounding assay (treating zebrafish embryos with drugs or control substances, wounding the embryo, staining the embryo, and counting neutrophils near the wound).
• summarize results into a figure and write a descriptive figure legend.
• perform appropriate statistical analysis.
• interpret results in a discussion that draws connections between the cytoskeleton and cell migration.
• put data into context by appropriately using information from journal articles in the introduction and discussion of a lab report.
• ### Predicting and classifying effects of insertion and deletion mutations on protein coding regions

Learning Objectives
Students will be able to:
• accurately predict effects of frameshift mutations in protein coding regions
• conduct statistical analysis to compare expected and observed values
• become familiar with accessing and using DNA sequence databases and analysis tools

Learning Objectives
Topics within Playon Words are grouped into “mini-games.” The Learning Objectives for each mini-game are as follows: Sentence Sensei
• Identify the best sentence variant from a list of options
• Identify and eliminate needless words
• Identify where and when to use different types of punctuation marks
• Identify and correct common grammar mistakes
Organization Optimizer
• Organize sentences in a logical order
• Describe the components of different sections of a scientific paper
• Identify the section of a scientific paper where a given sentence belongs
• Eliminate sentences which do not belong in a given writing sample
Science Officer Training
• Classify statements as scientific or non-scientific
• Identify which statements support a particular hypothesis or position
• Classify provided sentences (e.g. hypotheses vs. predictions, problems vs. experiments, results vs. discussion)
Reference Referee
• Compare and contrast different types (e.g. primary literature, review articles, popular literature etc.) and sources (PubMed, Web of Science, Google Scholar etc.) of scientific information
• Identify locations in texts where citations are needed
• Identify citations and/or references that are incorrect or missing key information
• Identify information that does not belong in the reference list (e.g. vendor information)
• ### Discovering Prokaryotic Gene Regulation by Building and Investigating a Computational Model of the lac Operon

Learning Objectives
Students will be able to:
• model how the components of the lac operon contribute to gene regulation and expression.
• generate and test predictions using computational modeling and simulations.
• interpret and record graphs displaying simulation results.
• relate simulation results to cellular events.
• describe how changes in environmental glucose and lactose levels impact regulation of the lac operon.
• predict, test, and explain how mutations in specific elements in the lac operon affect their protein product and other elements within the operon.
• ### Using Yeast to Make Scientists: A Six-Week Student-Driven Research Project for the Cell Biology Laboratory

Learning Objectives
• Learn about basic S. cerevisiae biology
• Use sterile technique
• Perform a yeast viability assay
• Use a spectrophotometer to measure growth of S. cerevisiae
• Perform a literature search
• Calculate concentrations of chemicals appropriate for S. cerevisiae
• Generate S. cerevisiae growth curves
• Troubleshoot experimental difficulties
• Perform statistical analysis
• Present findings to an audience