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Antibiotic Resistance Genes Detection in Environmental SamplesLearning ObjectivesAfter completing this laboratory series, students will be able to:
- apply the scientific method in formulating a hypothesis, designing a controlled experiment using appropriate molecular biology techniques, and analyzing experimental results;
- conduct a molecular biology experiment and explain the principles behind methodologies, such as accurate use of micropipettes, PCR (polymerase chain reaction), and gel electrophoresis;
- determine the presence of antibiotic-resistance genes in environmental samples by analyzing PCR products using gel electrophoresis;
- explain mechanisms of microbial antibiotic resistance;
- contribute data to the Antibiotic Resistance Genes Network;
- define and apply key concepts of antibiotic resistance and gene identification via PCR fragment size.
The Case of the Missing Strawberries: RFLP analysisLearning ObjectivesStudents will be able to:
- Describe the relationship of cells, chromosomes, and DNA.
- Isolate DNA from strawberries.
- Digest DNA with restriction enzymes.
- Perform gel electrophoresis.
- Design an experiment to compare DNAs by RFLP analysis.
- Predict results of RFLP analysis.
- Interpret results of RFLP analysis.
- Use appropriate safety procedures in the lab.