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Forensic Phylogenetics: Implementing Tree-thinking in a Court of LawLearning Objectives
- Students will be able to infer the topological and temporal relationships expected in an evolutionary tree (phylogeny) of a pathogen in the case of transmission from one host to the next.
- Students will be able to draw trees representing the transmission events from one host (patient zero) to multiple secondary patients.
Priority Setting in Public Health: A lesson in ethics and hard choicesLearning ObjectivesAt the end of this unit, students will be able to:
- Define the central distinction between public health and medicine
- Apply objectives of public health and individual medical care in a particular situation to identify potential areas of conflict in priority setting
- Apply moral theories of utilitarianism and deontology to a particular situation to identify the course of action proponents of each theory would see as morally justified
- Identify the range of morally justifiable actions that might be available to a health professional in a particular setting
- Choose from among a range of possible actions in a particular health situation and articulate the ethical principles that would justify that choice.
Using CRISPR-Cas9 to teach the fundamentals of molecular biology and experimental designLearning ObjectivesModule 1
- Generate a testable hypothesis that requires a creative design of reagents based on critical reading of and review of prior research.
- Demonstrate proficiency in using molecular cloning software to analyze, manipulate and verify DNA sequences.
- Predict the downstream effect on the mRNA and protein after successfully inserting a DNA repair template into the genome of a cell/organism.
- Compare and contrast the processes of DNA duplication and PCR.
- Demonstrate the ability to design primers to amplify a nucleotide sequence.
- Analyze and evaluate the results of DNA agarose gel electrophoresis.
- Identify the key features in genomic DNA, specifically those required for CRISPR-Cas9 mediated gene edits.
- Explain how compatible ends of DNA are used to produce recombinant DNA in a ligation reaction.
- Explain the chemical principles behind plasmid DNA purification from bacterial cultures.
- Devise a strategy to screen clones based on antibiotic selection and the mechanism of digestion by DNA endonucleases.
- Predict and evaluate the results of a diagnostic digest.
- Explain the chemical principles behind DNA purification using phenol-chloroform extraction and ethanol precipitation.
- Explain the key differences between DNA duplication and transcription.
- Demonstrate the ability to perform lab work with sterile technique.
- Compare and contrast the results of a non-denaturing vs. denaturing agarose gel.
- Evaluate the results of a denaturing agarose gel.
- Design and implement an experiment that tests the CRISPR-Cas9 principle.
- Predict the outcome of a successful in vitro Cas9 digest.
- Summarize important background information on gene of interest from analysis of primary literature.
- Produce figures and figure legends that clearly indicate results.
- Organize and construct a poster that clearly and professionally displays the important aspects of the lesson.
- Demonstrate understanding of the lesson by presenting a poster to an audience in lay terms, mid-level terms, or at an expert level.
- Demonstrate understanding of procedures by writing a formal materials and methods paper.
Evaluating the Quick Fix: Weight Loss Drugs and Cellular RespirationLearning Objectives
- Students will be able to explain how the energy from sugars is transformed into ATP via cellular respiration.
- Students will be able to predict an outcome if there is a perturbation in the cellular respiration pathway.
- Students will be able to state and evaluate a hypothesis.
- Students will be able to interpret data from a graph, and use that data to make inferences about the action of a drug.
Antibiotic Resistance Genes Detection in Environmental SamplesLearning ObjectivesAfter completing this laboratory series, students will be able to:
- apply the scientific method in formulating a hypothesis, designing a controlled experiment using appropriate molecular biology techniques, and analyzing experimental results;
- conduct a molecular biology experiment and explain the principles behind methodologies, such as accurate use of micropipettes, PCR (polymerase chain reaction), and gel electrophoresis;
- determine the presence of antibiotic-resistance genes in environmental samples by analyzing PCR products using gel electrophoresis;
- explain mechanisms of microbial antibiotic resistance;
- contribute data to the Antibiotic Resistance Genes Network;
- define and apply key concepts of antibiotic resistance and gene identification via PCR fragment size.
Using Place-Based Economically Relevant Organisms to Improve Student Understanding of the Roles of Carbon Dioxide,...Learning ObjectivesAt the end of this lesson, students will be able to:
- Describe the roles of light energy and carbon dioxide in photosynthetic organisms.
- Identify the effect of nutrients on the growth of photosynthetic organisms.
- Describe global cycles in atmospheric carbon dioxide levels and how they relate to photosynthetic organisms.