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Visits to the writing center and office hours provide students structured reflection and low-stakes feedback on...Learning Objectives
- Students will be able to write a lab report that contains a descriptive title, complete and concise abstract, substantive and relevant introduction that includes a testable hypothesis, descriptive methods, description and comparison of results of various testable groups, biological explanation of the results that reflect the testable hypothesis, a conclusion that contains societal implications or scientific impact, and references cited in the document.
- Students will be able to self-identify weaknesses and strengths of their writing.
- Students will understand how to utilize office hours and the writing center to receive feedback on their lab reports.
A flexible, multi-week approach to plant biology - How will plants respond to higher levels of CO2?Learning ObjectivesStudents will be able to:
- Apply findings from each week's lesson to make predictions and informed hypotheses about the next week's lesson.
- Keep a detailed laboratory notebook.
- Write and peer-edit the sections of a scientific paper, and collaboratively write an entire lab report in the form of a scientific research paper.
- Search for, find, and read scientific research papers.
- Work together as a team to conduct experiments.
- Connect findings and ideas from each week's lesson to get a broader understanding of how plants will respond to higher levels of CO2 (e.g., stomatal density, photosynthetic/respiratory rates, foliar protein concentrations, growth, and resource allocation).
Quantifying and Visualizing Campus Tree PhenologyLearning ObjectivesThe Learning Objectives of this lesson span across the entire semester.
- Observe and collect information on phenological changes in local trees.
- Become familiar with a database and how to work with large datasets.
- Analyze and visualize data from the database to test their hypotheses and questions.
- Develop a research proposal including empirically-driven questions and hypotheses.
- Synthesize the results of their analysis in the context of plant biodiversity and local environmental conditions.
Evaluating the Quick Fix: Weight Loss Drugs and Cellular RespirationLearning Objectives
- Students will be able to explain how the energy from sugars is transformed into ATP via cellular respiration.
- Students will be able to predict an outcome if there is a perturbation in the cellular respiration pathway.
- Students will be able to state and evaluate a hypothesis.
- Students will be able to interpret data from a graph, and use that data to make inferences about the action of a drug.
Air Quality Data Mining: Mining the US EPA AirData website for student-led evaluation of air quality issuesLearning ObjectivesStudents will be able to:
- Describe various parameters of air quality that can negatively impact human health, list priority air pollutants, and interpret the EPA Air Quality Index as it relates to human health.
- Identify an air quality problem that varies on spatial and/or temporal scales that can be addressed using publicly available U.S. EPA air data.
- Collect appropriate U.S. EPA Airdata information needed to answer that/those questions, using the U.S. EPA Airdata website data mining tools.
- Analyze the data as needed to address or answer their question(s).
- Interpret data and draw conclusions regarding air quality levels and/or impacts on human and public health.
- Communicate results in the form of a scientific paper.
A new approach to course-based research using a hermit crab-hydrozoan symbiosisLearning ObjectivesStudents will be able to:
- define different types of symbiotic interactions, with specific examples.
- summarize and critically evaluate contemporary primary literature relevant to ecological symbioses, in particular that between hermit crabs and Hydractinia spp.
- articulate a question, based on observations of a natural phenomenon (in this example, the hermit crab-Hydractinia interaction).
- articulate a testable hypothesis, based on their own observations and read of the literature.
- design appropriate experimental or observational studies to address their hypotheses.
- collect and interpret data in light of their hypotheses.
- problem-solve and troubleshoot issues that arise during their experiment.
- communicate scientific results, both orally and in written form.
Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Introductory Biology (identifying...Learning Objectives
- Describe how cells can produce proteins at the right time and correct amount.
- Diagram how a repressor works to reduce transcription.
- Diagram how an activator works to increase transcription.
- Identify a new promoter from literature and design a method to clone it and test its function.
- Successfully and safely manipulate DNA and Escherichia coli for ligation and transformation experiments.
- Design an experiment to verify a new promoter has been cloned into a destination vector.
- Design an experiment to measure the strength of a promoter.
- Analyze data showing reporter protein produced and use the data to assess promoter strength.
- Define type IIs restriction enzymes.
- Distinguish between type II and type IIs restriction enzymes.
- Explain how Golden Gate Assembly (GGA) works.
- Measure the relative strength of a promoter compared to a standard promoter.
Fly Exercise: A Simple Experiment to Test the Physiological Effects of Exercise on a Model OrganismLearning ObjectivesStudents will:
- demonstrate understanding of the concept and details of experimental design.
- perform an organic lipid extraction to determine total lipid content.
- quantify enzyme activity, as well as triglyceride, glucose, and glycogen concentrations.
- organize their collected data into spreadsheets for statistical analyses.
- interpret the results to gain insight on the varying effects exercise has on an organism's physiology.
- graphically present their results so that trends can be easily identified.
Follow the Sulfur: Using Yeast Mutants to Study a Metabolic PathwayLearning ObjectivesAt the end of this lesson, students will be able to:
- use spot plating techniques to compare the growth of yeast strains on solid culture media.
- predict the ability of specific met deletion strains to grow on media containing various sulfur sources.
- predict how mutations in specific genes will affect the concentrations of metabolites in the pathways involved in methionine biosynthesis.
Modeling the Research Process: Authentic human physiology research in a large non-majors courseLearning ObjectivesStudents will be able to:
- Read current scientific literature
- Formulate testable hypotheses
- Design an experimental procedure to test their hypothesis
- Make scientific observations
- Analyze and interpret data
- Communicate results visually and orally
The Avocado Lab: An Inquiry-Driven Exploration of an Enzymatic Browning ReactionLearning ObjectivesStudents will be able to:
- develop a testable research question and supportive hypothesis regarding the browning of damaged avocado flesh caused by the activity of avocado polyphenol oxidase (aPPO).
- design and execute a well-controlled experiment to test aPPO hypotheses.
- evaluate qualitative enzyme activity data.
- create a figure and legend to present qualitative data that tests multiple hypotheses and variables.
- search for and correctly cite primary literature to support or refute hypotheses.
- know the role of reducing reagents, pH, chelators, and temperature in reactions catalyzed by aPPO.
- explain why the effects of salt and detergent differ for aPPO experiments conducted in situ
- (in mashed avocado flesh) as compared to in vitro (on purified protein).
- discuss how substrate and cofactor availability affect aPPO reactions.
- describe how endogenous subcellular organization restricts aPPO reactions in a healthy avocado.
- evaluate food handling practices for fruits expressing PPO.
Using Pathway Maps to Link Concepts, Peer Review, Primary Literature Searches and Data Assessment in Large Enrollment...Learning Objectives
- Define basic concepts and terminology of Ecosystem Ecology
- Link biological processes that affect each other
- Evaluate whether the link causes a positive, negative, or neutral effect
- Find primary literature
- Identify data that correctly supports or refutes an hypothesis
Building Trees: Introducing evolutionary concepts by exploring Crassulaceae phylogeny and biogeographyLearning ObjectivesStudents will be able to:
- Estimate phylogenetic trees using diverse data types and phylogenetic models.
- Correctly make inferences about evolutionary history and relatedness from the tree diagrams obtained.
- Use selected computer programs for phylogenetic analysis.
- Use bootstrapping to assess the statistical support for a phylogeny.
- Use phylogenetic data to construct, compare, and evaluate the role of geologic processes in shaping the historical and current geographic distributions of a group of organisms.
Antibiotic Resistance Genes Detection in Environmental SamplesLearning ObjectivesAfter completing this laboratory series, students will be able to:
- apply the scientific method in formulating a hypothesis, designing a controlled experiment using appropriate molecular biology techniques, and analyzing experimental results;
- conduct a molecular biology experiment and explain the principles behind methodologies, such as accurate use of micropipettes, PCR (polymerase chain reaction), and gel electrophoresis;
- determine the presence of antibiotic-resistance genes in environmental samples by analyzing PCR products using gel electrophoresis;
- explain mechanisms of microbial antibiotic resistance;
- contribute data to the Antibiotic Resistance Genes Network;
- define and apply key concepts of antibiotic resistance and gene identification via PCR fragment size.
CURE-all: Large Scale Implementation of Authentic DNA Barcoding Research into First-Year Biology CurriculumLearning ObjectivesStudents will be able to: Week 1-4: Fundamentals of Science and Biology
- List the major processes involved in scientific discovery
- List the different types of scientific studies and which types can establish causation
- Design experiments with appropriate controls
- Create and evaluate phylogenetic trees
- Define taxonomy and phylogeny and explain their relationship to each other
- Explain DNA sequence divergence and how it applies to evolutionary relationships and DNA barcoding
- Define and measure biodiversity and explain its importance
- Catalog organisms using the morphospecies concept
- Geographically map organisms using smartphones and an online mapping program
- Calculate metrics of species diversity using spreadsheet software
- Use spreadsheet software to quantify and graph biodiversity at forest edges vs. interiors
- Write a formal lab report
- Extract, amplify, visualize and sequence DNA using standard molecular techniques (PCR, gel electrophoresis, Sanger sequencing)
- Explain how DNA extraction, PCR, gel electrophoresis, and Sanger sequencing work at the molecular level
- Trim and assemble raw DNA sequence data
- Taxonomically identify DNA sequences isolated from unknown organisms using BLAST
- Visualize sequence data relationships using sequence alignments and gene-based phylogenetic trees
- Map and report data in a publicly available online database
- Share data in a formal scientific poster
Knowing your own: A classroom case study using the scientific method to investigate how birds learn to recognize their...Learning Objectives
- Students will be able to identify and describe the steps of the scientific method.
- Students will be able to develop hypotheses and predictions.
- Students will be able to construct and interpret bar graphs based on data and predictions.
- Students will be able to draw conclusions from data presented in graphical form.
Bad Cell Reception? Using a cell part activity to help students appreciate cell biology, with an improved data plan and...Learning Objectives
- Identify cell parts and explain their function
- Explain how defects in a cell part can result in human disease
- Generate thought-provoking questions that expand upon existing knowledge
- Create a hypothesis and plan an experiment to answer a cell part question
- Find and reference relevant cell biology journal articles
Dynamic Daphnia: An inquiry-based research experience in ecology that teaches the scientific process to first-year...Learning ObjectivesStudents will be able to:
- Construct written predictions about 1 factor experiments.
- Interpret simple (2 variables) figures.
- Construct simple (2 variables) figures from data.
- Design simple 1 factor experiments with appropriate controls.
- Demonstrate proper use of standard laboratory items, including a two-stop pipette, stereomicroscope, and laboratory notebook.
- Calculate means and standard deviations.
- Given some scaffolding (instructions), select the correct statistical test for a data set, be able to run a t-test, ANOVA, chi-squared test, and linear regression in Microsoft Excel, and be able to correctly interpret their results.
- Construct and present a scientific poster.
Teaching Biodiversity with Museum Specimens in an Inquiry-Based LabLearning ObjectivesStudents completing this lab module will:
- Learn how to appropriately handle and measure museum specimens.
- Develop the necessary statistical skills to analyze museum specimen data.
- Become familiar with how to search an online museum database and integrate supplemental data with their own dataset.
- Strengthen scientific communication skills by presenting research to their peers.
- Demonstrate ability to investigate scientific questions and address obstacles that occur during data collection and integration.
- Increase proficiency in managing and using large datasets for scientific research.
- Make connections between natural history knowledge and morphology of organisms in developing and testing hypotheses.
You and Your Oral Microflora: Introducing non-biology majors to their “forgotten organ”Learning ObjectivesStudents will be able to:
- Explain both beneficial and detrimental roles of microbes in human health.
- Compare and contrast DNA replication as it occurs inside a cell versus in a test tube
- Identify an unknown sequence of DNA by performing a BLAST search
- Navigate sources of scientific information to assess the accuracy of their experimental techniques