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Follow the Sulfur: Using Yeast Mutants to Study a Metabolic PathwayLearning ObjectivesAt the end of this lesson, students will be able to:
- use spot plating techniques to compare the growth of yeast strains on solid culture media.
- predict the ability of specific met deletion strains to grow on media containing various sulfur sources.
- predict how mutations in specific genes will affect the concentrations of metabolites in the pathways involved in methionine biosynthesis.
Antibiotic Resistance Genes Detection in Environmental SamplesLearning ObjectivesAfter completing this laboratory series, students will be able to:
- apply the scientific method in formulating a hypothesis, designing a controlled experiment using appropriate molecular biology techniques, and analyzing experimental results;
- conduct a molecular biology experiment and explain the principles behind methodologies, such as accurate use of micropipettes, PCR (polymerase chain reaction), and gel electrophoresis;
- determine the presence of antibiotic-resistance genes in environmental samples by analyzing PCR products using gel electrophoresis;
- explain mechanisms of microbial antibiotic resistance;
- contribute data to the Antibiotic Resistance Genes Network;
- define and apply key concepts of antibiotic resistance and gene identification via PCR fragment size.
You and Your Oral Microflora: Introducing non-biology majors to their “forgotten organ”Learning ObjectivesStudents will be able to:
- Explain both beneficial and detrimental roles of microbes in human health.
- Compare and contrast DNA replication as it occurs inside a cell versus in a test tube
- Identify an unknown sequence of DNA by performing a BLAST search
- Navigate sources of scientific information to assess the accuracy of their experimental techniques
Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Introductory Biology (identifying...Learning Objectives
- Describe how cells can produce proteins at the right time and correct amount.
- Diagram how a repressor works to reduce transcription.
- Diagram how an activator works to increase transcription.
- Identify a new promoter from literature and design a method to clone it and test its function.
- Successfully and safely manipulate DNA and Escherichia coli for ligation and transformation experiments.
- Design an experiment to verify a new promoter has been cloned into a destination vector.
- Design an experiment to measure the strength of a promoter.
- Analyze data showing reporter protein produced and use the data to assess promoter strength.
- Define type IIs restriction enzymes.
- Distinguish between type II and type IIs restriction enzymes.
- Explain how Golden Gate Assembly (GGA) works.
- Measure the relative strength of a promoter compared to a standard promoter.
Dynamic Daphnia: An inquiry-based research experience in ecology that teaches the scientific process to first-year...Learning ObjectivesStudents will be able to:
- Construct written predictions about 1 factor experiments.
- Interpret simple (2 variables) figures.
- Construct simple (2 variables) figures from data.
- Design simple 1 factor experiments with appropriate controls.
- Demonstrate proper use of standard laboratory items, including a two-stop pipette, stereomicroscope, and laboratory notebook.
- Calculate means and standard deviations.
- Given some scaffolding (instructions), select the correct statistical test for a data set, be able to run a t-test, ANOVA, chi-squared test, and linear regression in Microsoft Excel, and be able to correctly interpret their results.
- Construct and present a scientific poster.
Linking Genotype to Phenotype: The Effect of a Mutation in Gibberellic Acid Production on Plant GerminationLearning ObjectivesStudents will be able to:
- identify when germination occurs.
- score germination in the presence and absence of GA to construct graphs of collated class data of wild-type and mutant specimens.
- identify the genotype of an unknown sample based on the analysis of their graphical data.
- organize data and perform quantitative data analysis.
- explain the importance of GA for plant germination.
- connect the inheritance of a mutation with the observed phenotype.