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Cell Biology

  • Structure of protein ADA2

    Understanding Protein Domains: A Modular Approach

    Learning Objectives
    • Students will be able to compare protein sequences and identify conserved regions and putative domains.
    • Students will be able to obtain, examine, and compare structural models of protein domains.
    • Students will be able to interpret data on protein interactions (in vitro pull-down and in vitro and in vivo functional assays)
    • Students will be able to propose experiments to test protein interactions.
  • Neutrophils in a Danio rerio Embryo. Student-generated picture of a wounded zebrafish embryo that was stained to show the neutrophils (small black dots) that had migrated toward the wound site on the fin.

    Inexpensive Cell Migration Inquiry Lab using Zebrafish

    Learning Objectives
    Students will:
    • formulate a hypothesis and design an experiment with the proper controls.
    • describe the steps involved in the zebrafish wounding assay (treating zebrafish embryos with drugs or control substances, wounding the embryo, staining the embryo, and counting neutrophils near the wound).
    • summarize results into a figure and write a descriptive figure legend.
    • perform appropriate statistical analysis.
    • interpret results in a discussion that draws connections between the cytoskeleton and cell migration.
    • put data into context by appropriately using information from journal articles in the introduction and discussion of a lab report.
  • Sample Student Growth Curve. This image shows a yeast growth curve generated by a student in our lab, superimposed on an image of Saccharomyces cerevisiae cells.

    Using Yeast to Make Scientists: A Six-Week Student-Driven Research Project for the Cell Biology Laboratory

    Learning Objectives
    • Learn about basic S. cerevisiae biology
    • Use sterile technique
    • Perform a yeast viability assay
    • Use a spectrophotometer to measure growth of S. cerevisiae
    • Perform a literature search
    • Calculate concentrations of chemicals appropriate for S. cerevisiae
    • Generate S. cerevisiae growth curves
    • Troubleshoot experimental difficulties
    • Perform statistical analysis
    • Present findings to an audience