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Using a Sequential Interpretation of Data in Envelopes (SIDE) approach to identify a mystery TRP channelLearning Objectives
- Students will be able to analyze data from multiple experimental methodologies to determine the identity of their "mystery" TRP channel.
- Students will be able to interpret the results of individual experiments and from multiple experiments simultaneously to identify their "mystery" TRP channel.
- Students will be able to evaluate the advantages and limitations of experimental methodologies presented in this lesson.
Inexpensive Cell Migration Inquiry Lab using ZebrafishLearning ObjectivesStudents will:
- formulate a hypothesis and design an experiment with the proper controls.
- describe the steps involved in the zebrafish wounding assay (treating zebrafish embryos with drugs or control substances, wounding the embryo, staining the embryo, and counting neutrophils near the wound).
- summarize results into a figure and write a descriptive figure legend.
- perform appropriate statistical analysis.
- interpret results in a discussion that draws connections between the cytoskeleton and cell migration.
- put data into context by appropriately using information from journal articles in the introduction and discussion of a lab report.
Investigating the Function of a Transport Protein: Where is ABCB6 Located in Human Cells?Learning ObjectivesAt the end of this activity students will be able to:
- describe the use of two common research techniques for studying proteins: SDS-PAGE and immunoblot analysis.
- determine a protein’s subcellular location based on results from: 1) immunoblotting after differential centrifugation, and 2) immunofluorescence microscopy.
- analyze protein localization data based on the limitations of differential centrifugation and immunofluorescence microscopy.
Cell Signaling Pathways - a Case Study ApproachLearning Objectives
- Use knowledge of positive and negative regulation of signaling pathways to predict the outcome of genetic modifications or pharmaceutical manipulation.
- From phenotypic data, predict whether a mutation is in a coding or a regulatory region of a gene involved in signaling.
- Use data, combined with knowledge of pathways, to make reasonable predictions about the genetic basis of altered signaling pathways.
- Interpret and use pathway diagrams.
- Synthesize information by applying prior knowledge on gene expression when considering congenital syndromes.