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Biochemistry And Molecular Biology

  • A three-dimensional model of methionine is superimposed on a phase contrast micrograph of Saccharomyces cerevisiae from a log phase culture.

    Follow the Sulfur: Using Yeast Mutants to Study a Metabolic Pathway

    Learning Objectives
    At the end of this lesson, students will be able to:
    • use spot plating techniques to compare the growth of yeast strains on solid culture media.
    • predict the ability of specific met deletion strains to grow on media containing various sulfur sources.
    • predict how mutations in specific genes will affect the concentrations of metabolites in the pathways involved in methionine biosynthesis.
  • The Flygometer 2.0: The photo is of the Fly Treadmill used in this experiment.

    Fly Exercise: A Simple Experiment to Test the Physiological Effects of Exercise on a Model Organism

    Learning Objectives
    Students will:
    • demonstrate understanding of the concept and details of experimental design.
    • perform an organic lipid extraction to determine total lipid content.
    • quantify enzyme activity, as well as triglyceride, glucose, and glycogen concentrations.
    • organize their collected data into spreadsheets for statistical analyses.
    • interpret the results to gain insight on the varying effects exercise has on an organism's physiology.
    • graphically present their results so that trends can be easily identified.