Topic
DNA - Information Storage [GENOMICS]
Framing Question
How can bioinformatics tools be employed to analyze genetic information?
Learning Objectives
- Calculate the alignment score between two DNA sequences using a provided scoring matrix.
- Perform a BLASTN search and interpret the results.
- Explain the BLASTN algorithm for nucleotide sequence information.
- Interpret the biological significance of an e-value.
- Annotate a prokaryotic gene (derive a model).
- Annotate a eukaryotic gene (derive a model).
- Create and interpret a multiple sequence alignment (e.g., T-COFFEE, MUSCLE, etc.).
- For a genomic region of interest (e.g., the neighborhood of a particular gene), use a genome browser to view nearby genes, transcription factor binding regions, epigenetic information, etc.
- Describe Hidden Markov Models and how they can be used to assess motifs.
Related Articles
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- A CURE-based approach to teaching genomics using mitochondrial genomes
- A Hands-on Introduction to Hidden Markov Models
- An Introduction to Eukaryotic Genome Analysis in Non-model Species for Undergraduates: A tutorial from the Genome Consortium for Active Teaching
- An undergraduate bioinformatics curriculum that teaches eukaryotic gene structure
- Exploration of the Human Genome by Investigation of Personalized SNPs
- Homologous chromosomes? Exploring human sex chromosomes, sex determination and sex reversal using bioinformatics approaches
- Mapping a Mutation to its Gene: The "Fly Lab" as a Modern Research Experience
- Predicting and classifying effects of insertion and deletion mutations on protein coding regions
- Sequence Similarity: An inquiry based and "under the hood" approach for incorporating molecular sequence alignment in introductory undergraduate biology courses
