Energy is required and transformed in biological systems
How do enzymes catalyze biological reactions?
- Identify the factors contributing to the activation energy of a reaction.
- Explain transition state stabilization.
- Calculate the rate enhancement of an enzyme-catalyzed reaction.
- Explain what a substrate is in terms of being a reactant.
- Differentiate between the activation energy, the free energy and standard free energy of a reaction.
- Use kinetic parameters to compare enzymes.
- Distinguish the different forms of catalytic inhibition and explain how and why they differ.
- Quantitatively model how catalyzed reactions occur and calculate kinetic parameters of enzymes from experimental data.
- Explain how catalytic parameters vary as one varies substrate or enzyme concentration.
- Interpret the physical meaning of various kinetic parameters and describe the underlying assumptions and conditions (such as steady state or equilibrium) on which different parameters depend