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What is the role of noncovalent intermolecular interactions?

Topic

Macromolecular Structure Determines Function and Regulation

Framing Question

What is the role of noncovalent intermolecular interactions?

Learning Objectives

  • Discuss the impact of specificity or affinity changes on biological function and any potential evolutionary impact.
  • Discuss the various methods that can be used to determine affinity and stoichiometry for a ligand-macromolecule complex and relate the results to both thermodynamic and kinetic data
  • Discuss the interactions between a variety of biological molecules (including proteins, nucleic acids, lipids, carbohydrates and small organics, etc.) and describe how these interactions impact specificity or affinity leading to changes in biological function.
  • Predict the effects of either mutation or ligand structural change on the affinity of binding and design appropriate experiments to test their predictions.
  • Discuss the relationship between the temperature required for denaturation (Tm) and macromolecular structure.

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